Part:BBa_K3747137

napEDABC (Periplasmic nitrate reductase) Pseudomonas stutzeri JM300

Nap is a dissimilatory, periplasmic nitrate reductase which is expressed under aerobic conditions and is required for aerobic denitrification [1]. Nap is the first enzyme in the denitrification pathway and reduces nitrate (NO₃^-) to nitrite (NO₂^-). To establish denitrification in Pseudomonas putida EM42, we expressed this construct and tested its activity to convert NO₃^- into NO₂^-.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 852
  Illegal EcoRI site found at 2205
  Illegal SpeI site found at 2156
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 852
  Illegal EcoRI site found at 2205
  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal SpeI site found at 2156
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 852
  Illegal EcoRI site found at 2205
  Illegal BglII site found at 1846
  Illegal BglII site found at 3859
  Illegal XhoI site found at 411
  Illegal XhoI site found at 1527
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 852
  Illegal EcoRI site found at 2205
  Illegal SpeI site found at 2156
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 852
  Illegal EcoRI site found at 2205
  Illegal SpeI site found at 2156
  Illegal NgoMIV site found at 219
  Illegal NgoMIV site found at 1201
  Illegal NgoMIV site found at 1971
  Illegal NgoMIV site found at 2818
  Illegal NgoMIV site found at 3121
  Illegal NgoMIV site found at 3627
  Illegal AgeI site found at 663
  Illegal AgeI site found at 2667
  
• 1000
  COMPATIBLE WITH RFC[1000]

Reference

[1] Ji, B., Yang, K., Zhu, L., Jiang, Y., Wang, H., Zhou, J., & Zhang, H. (2015). Aerobic Denitrification: A Review of Important Advances of the Last 30 Years. Biotechnology and Bioprocess Engineering, 20, 643–651. https://doi.org/10.1007/s12257-015-0009-0